Devise an online lesson Assignment Example | Topics and Well Written Essays - 500 words

Devise an online lesson - Assignment Example This will greatly assist them in understanding and sharing of newly introduced concepts The plan will try to satisfy all the course objectives by helping students to expand their knowledge and grasp such key terminologies as pronunciation, nouns, verbs, tenses adverbs, vocabularies and many more others. The plan will also help the students appreciate English as a language and demystify any stereotypic beliefs surrounding it. The quote will depend on the topic of the day. Most quotes will be drawn from great thinkers like Aristotle, Hobbes and Rousseau. The quotes will help the students focus on the topic of the day and motivate them to complete their assignments within the provided time frame. Students will be instructed on which online methods to use during teaching. Some classes like pronunciation will require computer conferencing while others like tackling assignments will require World Wide Web. The three media above have been chosen because they will increase the speed at which students will converse with their instructor. They will also give them an opportunity to interact among themselves thus creating room form lively forums. Online communication is good because it helps the students open up and tell the instructor what they would have otherwise never said in an open classroom (Anderson, 2008). It also saves time and resources as students will not be required to appear physically in class. By so doing, they will be able to engage in other activities like spending time with their families and attending their jobs without any worry. The methods have also been chosen because of their ability to help students from different cultures interact and appreciate one another’s

Chromatography Technique for Purification

Chromatography Technique for Purification Introduction Column packing is an integral part of the purification process in the manufacture of biologics. The goal is always to ensure reproducibility with regard to the technique to be used. Manual packing might sometimes involve several attempts to get an optimal packing as this would affect the purification process. The resin to be used in the packing process has to be well defined as it could impact on the flow rate which could lead to a reduced through put. The mobile phase is also important as the rationale behind the choice would be looking for a solvent that can pack the resin more tightly. In any chromatography technique that has to be utilised whether for the need to capture, purify or polish four integral parameters which includes, resolution, speed, capacity and recovery are always considered. Resolution is the most difficult to achieve especially during the polishing stage were impurities can be construed as having similar properties to the product. The efficiency of the column packing thus has a significant role to play on this basis as it is a good measure how consistently the column can perform. 1.1 Material: As per SOP 1.2 Key Instrument Components Bubble Trap BT1 Filter Housing F1 Inlet / Outlet Valves V001 V101 UV Sensor – QIR4 pH Probe – QIR3 Conductivity Meter – QIR2 Drain – V102 Column top and bottom connections – DN Â ¼ 1.3 Preparation of column for packing: As per SOP TRG-DSP-052. 1.4 Determination of % slurry: Procedure was followed as per instruction manual Results: Table 1 Results: Table 2 r=5cm h= 15cm 1.178 Volume of gravity settled resin for packing (Vgs) 1.178 x 1.33 1.56674 Slurry volume needed from container (SVc) (To give you the desired amount of gravity settled resin) (c) Vgs x 100 % slurry in container = 1.55574 X 100/66 % Slurry in container = 66% 2.37384 Adjusting the slurry to the desired % concentration for packing Slurry volume required for packing (SVp) Vgs x 100 % slurry for packing = 1.56674 X 100/ 70 % Slurry for packing = 70% 2.2382 Volume packing buffer to add SVp – SVc = 2.2382- 2.37384 -0.13564 Volume to be added is thus 0.13564 Litre Calculations: Number of theoretical = N Where VR = volume eluted from the start of sample application to the peak maximum = 8CM W h = peak width measured as the width of the recorded peak at half of the peak height = 0.5CM N = 5.54 X Â ² Number of theoretical plates = 1418.24 HETP = L/N Where L = Bed height (cm) As we already know N (1418.24) HETP = 15/ 1418.24 = 0.0105765 Asymmetry factor (AS) = b/a Where a = 1st half peak width at 10% of peak height (0.5cm) b = 2nd half peak width at 10% of peak height (0.5cm) = 0.5/0. = 1 As rule of the thumb a good HETP value should be at least two to three times the average matrix bead size and normally in the range of 0.0018cm to 0.035cm. Looking at our column our HETP value was approximately 0.0106 and our bead has a pore size of about 40 microns which equates to 0.004cm and this is about 3 times our HETP. Our column can thus be confirmed to be within the acceptable range. In the event that our column is not within the acceptable range several factors such as the following can be construed as being responsible. Uneven packing of the column or exceeding the optimal packing flow rate The possibility of channelling in the bed Inadequate CIP can also be a factor as this can result in a build-up of contamination in the column thus impacting on flow and other performance determinants of the column. Cleaning is also important to wash the matrix storage solution which is an unwanted entity during packing. Air entrapment prevalence of air bubbles can also affect the HETP values. The possibility of a void being present at the inlet can also be a contributing factor to the value of HETP not being within specification The choice of resin is also very important as the possibility of the solute reacting with the resin can result in an ambiguous HETP value. Peak asymmetry is an important measure in the determination of column efficiency and in conjunction with the HETP value is always used in the calibration of a new or existing column. The gold standard is the ability to achieve an asymmetry value of 1 although the acceptable range is normally between 0.8 and 1.2. An asymmetry value greater than 1 indicates the prevalence of extensive tailing while an asymmetry value less than 1 indicates extensive fronting. Taking our packed column into consideration, our asymmetry value from the chromatogram was 1 and one would generally thus expect a high efficiency and resolution. However, in the event of our column not being within the acceptable asymmetry value the following reason are the possible causes. Extensive tailing which is characterised by an asymmetry value greater than 1 as mentioned earlier can be a reason. This factor is a result of column being packed too loosely and it can be observed from the chromatogram by the peak tailing gradually. Extensive fronting is also a possible cause and it is characterised by an asymmetry factor less than 1 which is normally as a result of the column being packed tightly and would be noticeable on the chromatogram by the peaks developing slowly. Possible causes of resin/column deterioration and their remedies Temperature the resins have a temperature range that is normally specified by the manufacturers and a usually high temperature can cause irreversible damage due to loss of functional groups. It is thus important that operation should always within the optimal ranges and bearing in mind the fact that temperature maxima is only for indication. Oxidation – The functional groups are also attached by oxidation and on this basis one has to ensure that oxidants such as hydrochloric acid , nitric acid are not utilised in the cleaning regime as they can accelerate oxidation which damages the polymer crosslink Fouling apart from impacting on performance of the column can also cause irreversible damage to the resin. Fouling can result due to the presence of iron and silica for this reason special attention has to be paid to the type of resin to be used as prevention they say is better than cure. Drying out and cracking of the resin is also an important reason for column deterioration and this can be remedied by ensuring that the column is well equilibrated. High pressure -The build-up can also cause damage to the resin/column and it could be as a result of flow path restriction due to dirty or worn bed support. Manufacturer’s specification should always be adhered to in ensuring an optimal usage of the resin. The life span of the resin/column should also be taken into account and usage should always be as specified by the manufacturer. Harsh elution is another factor that is responsible for irreversible damages to resin/column. Every resin has a pH range that is optimal and this should be adhered to strictly. Conclusion: The practical experience was so interesting and brought the protein purification lectures received into perspective. A better understanding of the process was developed and the practical knowledge is quite adaptive to the day to day operation in a typical Biopharmaceutical plant.

Civil Rights Timeline: Jan. 15, 1929 - Dec. 21, 1956 :: American Civil Rights

Civil Rights Timeline: Jan. 15, 1929 - Dec. 21, 1956 Jan. 15, 1929 - Dr. King is born - Born on Jan. 15, 1929, in Atlanta, Ga., he was the second of three children of the Rev. Michael (later Martin) and Alberta Williams King. Sept. 1, 1954 - Dr. King becomes pastor - In 1954, King accepted his first pastorate--the Dexter Avenue Baptist Church in Montgomery, Ala. He and his wife, Coretta Scott King, whom he had met and married (June 1953) while at Boston University. Dec. 1, 1955 - Rosa Parks defies city segregation - Often called "the mother of the civil rights movement," Rosa Louise McCauley Parks, b. Tuskegee, Ala., Feb. 4, 1913, sparked the 381-day Montgomery bus boycott that led to a 1956 Supreme Court order outlawing discriminatory practices on Montgomery buses. In December 1955, returning home from her assistant tailor job in Montgomery, Parks refused a bus driver's order to surrender her seat to a white man. She was jailed and fined $14. Dec. 5, 1955 - Montgomery bus boycott- Although precipitated by the arrest of Rosa Parks, the Montgomery Bus Boycott of 1955-56 was actually a collective response to decades of intimidation, harassment and discrimination of Alabama's African American population. By 1955, judicial decisions were still the principal means of struggle for civil rights, even though picketing, marches and boycotts sometimes punctuated the litigation. The boycott, which lasted for more than a year, was almost 100 percent effective. Dec. 21, 1956 - Bus segregation declared illegal - The boycott's succeeded in desegregating public facilities in the South and also in obtaining civil rights legislation from Congress.  Civil Rights Timeline Sept. 24, 1957 - May 2, 1963 Sept. 24, 1957 - School integration - In September 1957 the state received national attention when Gov. Orval E. Faubus (in office 1955-67) tried to prevent the integration of Little Rock Central High School. President Dwight D. Eisenhower quickly intervened, in part by sending federal troops to Little Rock, and several black students were enrolled at Central High School. Aug. 19, 1958 - Student sit-ins - In spite of the events in Little Rock or Montgomery, or Supreme Court decisions, segregation still pervaded American society by 1960. While protests and boycotts achieved moderate successes in desegregating aspects of education and transportation, other facilities such as restaurants, theaters, libraries, amusement parks and churches either barred or limited access to African Americans, or maintained separate, invariably inferior, facilities for black patrons.

American Express Diversity

American Express continues to be the one biggest company for selling financial products and is one of the most stable. Established in 1850 in New York, American Express Company was among the first and most successful express delivery businesses to arise during the rapid westward expansion of the United States (American Express Website, 2008). With over 150 years of experience in handling the business, the company is continually eyeing global expansion and international growth. The company employs a large number of employees from across Asia and Northern America.That includes different nationalities, cultures, with different ideologies, sects and religion. This shows how diverse American Express is. Diversity is generally defined as acknowledging, understanding, accepting, valuing, and celebrating differences among people with respect to age, class, ethnicity, gender, physical and mental ability, race, sexual orientation, spiritual practice, and public assistance status (Green, et. al , 2002). Now, how does American Express manages this kind of corporate setup? This is where leaders emerge.The company trains potential leaders through series of leadership enhancing courses with its goals, objectives and target outputs and film showing about the importance of working in a team. But first, leaders are asked on their knowledge of every individual employee’s human and cultural diversity. A Leader should be a motivational one, the kind who could open employees mind to working towards diversity. One must also show the big picture of its importance to the growth of the company and who will reap its benefits.Every conflict of ideas or differences must be resolved immediately through the ombudsmen department. All of these are needed to create synergies to maximize profit. American Express has created the Global Diversity and Inclusion. This will bridge the gap rooting from the immediate conflict on a local are to escalate into a network scene and the identification of diversity related arguments. All are monitored from the smallest department. Launching sports festivities and lunch and learn activities are effective ways of interacting all employees.Employees are allowed to express their opinions in the company. The workplace operates with respect for each other. The knowledge that leaders get from training modules are translated to the employees. The management sees to it that diversity is always practiced in the company. All are treated equally and if not, immediate actions are imposed. Although, discrimination still exists, all are advised to take it away. All opinions will be heard. Hispanics, Asians, Blacks, Muslims and employees of other religion work for the company.They may have their groups according to their faith, but all are working towards the corporate goals. Differences on faith are strongly respected. This shows how diverse the working place is. The company strives hard in leveraging these cultural gaps. Employees are all given equal opportunities as to be promoted or as to climb the corporate ladder. No one is given preferential treatment based on their ethnicity or color. This has been the most important practice of the company to promote diversity.Anyone who noticed that such wrongdoings can file complaints in the ombudsman department or any complaint centers. The importance of diversity reflects the company’s success. The operations in other countries are stronger for local employees talk to customers in their national language and know how to relate to them. This help the company expand faster and grow stronger. This also strengthens one of American Express corporate values, TEAMWORK, we work together, across boundaries, to meet the needs of our customers and to help the company win (American Express Website, 2008).References: American Express Website. (2008). Becoming American Express: 150+ Years of Reinvention and Customer Service. Retrieved, October 5, 2008, from http://home3. americanexpre ss. com/corp/os/history. asp Green, Kelli A, Mayra Lopez, Allen Wysocki, and Karl Kepner. (July, 2002). Diversity in the Workplace: Benefits, Challenges, and the Required Managerial Tools. Retrieved, October 5, 2008, from http://edis. ifas. ufl. edu/HR022 American Express Website. (2008). Our Values, Teamwork. Retrieved, October 5, 2008, from http://home3. americanexpress. com/corp/os/values. asp

Housekeeping by Marilynne Robinson: An Analysis Essay

Throughout chapters one and two of Housekeeping by Marilynne Robinson, the narrator of the novel, Ruth, compares and contrasts the parenting styles of both her grandmother and great aunts towards her sister Lucille and herself. The girls have been between two different parenting styles, one that was caring but cautious, and another that was well-meaning but indifferent. The styles of parenting presented to Lucille and Ruth by the women in their life play an important role in how the girls will learn receive love and care in their lives. They have learned so far that love is fragile and true relationships cannot be forged in a day-dream manner. Slyvia’s parenting style is the best example of the day-dream concept. After losing her husband in a train accident, Slyvia (the girl’s grandmother), tries her best to nurture her granddaughters in an orderly but distant manner. The years that she raised her girls in this fashion was effective in the present, but the result of her surface level interactions with her daughters left her estranged from them in her old age. Ruth and Lucille’s grandmother sees her opportunity to take care of them after their mother’s death as a second chance to fix her past mistakes as a mother. Their grandmother believes that she never really fostered a strong bond with her daughters, she was able to learn her daughters’ routines in the house and have them do their chores in an orderly manner, but she never got to know who they were as people, and most importantly â€Å"She had never taught them to be kind to her. † (Pg. 19) This quote emphasizes the idea that Slyvia never taught her daughters how to truly love. I think it is the lack of intimacy she shared with her daughters that she regrets the most. Because Slyvia regrets her inability to know her daughters as people, she now wishes to distance herself from her daughter’s inner thoughts, most likely from the fear of shock. â€Å"Perhaps she did not wish to learn by indirection what Helen did not wish to tell her. † (Pg. 20) Lucille and Ruth’s mother was proven to be unstable, and her suicide shed light on the internal suffering she must have been enduring before she killed herself. Slyvia, I feel, does not want to ponder the idea that her daughter was severely unhappy, especially given the fact that she felt her time spent with her daughters in their youth was full of serenity. Ruth realizes that their grandmother is over-consciousness but she believes it’s due to her heightened sense of perception. â€Å"I think that she was aware of too many things, having no principle for selecting the more from the less important, and that her awareness could never be diminished, since it was among the things she had thought of as familiar that this disaster had taken shape. † (Pg. 5) Ruth and Lucille’s grandmother has always been cautious in her parenting style because she is afraid to lose her charges like she lost her husband, fleetingly and without notice. And, it is this nervous cautiousness that has harbored a sense of quiet awareness and self-duty both her daughters and granddaughters. The self-duty becomes even more necessary for the girls to carry-on with their lives after their grandmother passes. They are handed off to their great aunts Lilly and Nona after Sylvia’s death, and their awkward pretentious sense of nurturing leaves an even bigger sense of distance in mother figures for the girls. Lilly and Nona had no genuine compassion for their god nieces; they simply were taking care of the girls because it was the dutiful thing to do. An example of their stiff compassion can be shown on page 29 of the novel â€Å"They were, though maiden ladies, of a buxomly maternal appearance that contrasted oddly with their brusque, unpracticed pats and kisses. † The quote is describing the two aunts as motherly appearing figures, but in contrast they were actually very far from being as warm and affectionate as true mothers are capable of being. Ruth’s conclusion after staying a few days with her aunts of their temperaments can be seen in the following quote. â€Å"Lily and Nona, I think, enjoyed nothing except habit and familiarity, the precise replication of one day in the next. † (Pg. 32) The two aunt’s like routine and they dislike anything disrupting that routine especially children. They have no patience or energy for doing new things, and they are not willing to adapt to Ruth and Lilly’s home town wholly, they often talk about moving the girls back to their own home. The contrast in the grandmother’s and the great aunt’s parenting style is self-explanatory. The grandmother’s affection was overzealous while the great aunts’ display is hollow. The girls are affected in a way that paints their image of love, their grandmother taught them to be intuitive of other’s feelings, while their great aunts’ have shown them that people are not always selfless. The most important thing the girls have yet to experience in a parent/child relationship however is a true bond of love and full awareness of who the other is as a person. The girls so far have been taught to feign emotions that are proper, and to show surface level respect for their mother figures.